AAPE is mixture of refined growth factors extracted from human adipose.

To promote hair growth and scalp health by outstanding ingredients.

  • US FDA, CFDA Approval
  • PCPC US registrationoutnumbered only by upper-respiratory infections.
  • 15 International SCI journals
  • 28 Patents registration worldwide

DESCRIPTION

AAPE is mixture of refined growth factors extracted from human adipose.
AAPE is mixture of refined growth factors extracted from human adipose-derived stem cells conditioned media and induces proliferation of dermal papilla cells of human hair follicles to make hair regrow twice faster.

 

PROTOCOL

AAPE applies to the various types of treatment such as Micro-needle, Fractional Laser and Iontophoresis helping AAPE to maximize penetration of active ingredients of AAPE into skin.

AAPE applies to the various types of treatment such as Micro-needle, Fractional Laser and Iontophoresis helping AAPE to maximize penetration of active ingredients of AAPE into skin.

To schedule a free consultation of our AAPE treatment,

AAPE / ADSC-CM Treatment Is Derived From Adipose

The use of AAPE, “fat” or adipose-derived stem cells conditioned media or ADSC-CM has been used successfully in the treatment of female hair loss. The non-invasive treatment is described in the following journal article, including a new study and scientific analysis of hair growth measurements as well as photos of the results, as published in the International Journal of Dermatology.

In another related treatment, adipose or “fat” tissue can also be obtained directly from the patient through a mini-liposuction style procedure. This adipose or fat tissue contains large numbers of MSCs Mesenchymal Stem Cells which are located within the SVF or Stromal Vascular Fraction obtained from liposuction.  A simple, sterile, closed-system process is used to separate and obtain the autologous cSVF or cellular stromal vascular fraction, which contains these powerful regenerative stem and stromal cells, progenitor cells, growth factors and many other powerful biologic components.  This biocellular regenerative treatment can be used with PRP or Platelet Rich Plasma. Like PRP, recent scientific literature supports the use of adipose-derived stem cells to help enhance hair growth from weak hair follicles.

Adipose derived stem cell treatments can be used as a stand-alone treatment or in conjunction with other therapies for hair regrowth like PRP, laser therapy, medications, as well as hair transplantation. A growing number of clinical trials in Florida on adipose or fat-derived stem cells for hair growth can be found listed at clinicaltrials.gov, however many other studies are not publicly listed.

 

Clinical use of conditioned media of adipose tissue-derived stem cells in female pattern hair loss: a retrospective case series study

Hyoseung Shin1, MD, Hyeong Ho Ryu2, MD, Ohsang Kwon2, MD, PhD, Byung-Soon Park3,*, MD, PhD, and Seong Jin Jo1,*, MD, PhD

1Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, South Korea, 2Department of Dermatology, Seoul National University College of Medicine, Seoul, South Korea, and 3Cellpark Clinic, Seoul, South Korea

*These authors contributed equally to this work.

Conflicts of interest: None.

International Journal of Dermatology 2015, 54, 730–735  –   International Society of Dermatology

Abstract

Background: Female pattern hair loss (FPHL) is a common disorder but presents severe psychosocial problems in many female patients. Adipose tissue-derived stem cells (ADSCs) and conditioned media of ADSCs (ADSC-CM) are reported to promote hair growth in vitro. However, there are no clinical reports on the treatment of alopecia using ADSC-CM.

Objectives: This study evaluates our clinical experience in the use of ADSC-CM for the treatment of FPHL.

Methods: A retrospective, observational study of outcomes in 27 patients with FPHL treated with ADSC-CM was performed. To evaluate the efficacy of the treatment, patients’ medical records and phototrichographic images were analyzed.

Results: The application of ADSC-CM showed efficacy in treating FPHL after 12 weeks of therapy. Hair density increased from 105.4 to 122.7 hairs/cm2 (P < 0.001). Hair thickness increased from 57.5 lm to 64.0 lm (P < 0.001). None of the patients reported severe adverse reactions.

Conclusions: The application of ADSC-CM is a potential treatment option for FPHL.

STEM CELL AAPE HAIR TREATMENT

INTRODUCTION

Stem cells are immature precursor cells characterized by capacities of self-renewal and multi-lineage differentiation. Mesenchymal stem cells (MSCs), which represent a type of stem cell, were first identified in bone marrow, but the existence of MSCs in connective tissue has been reported.1 It was recently found that MSCs are abundant in adipose tissue, which is easily accessible. The yield of MSCs from adipose tissue is approximately 40-fold greater than that from bone marrow.2 Such characteristics can make it easy to investigate and use MSCs in clinical fields. Adipose tissue-derived stem cells (ADSCs) produce growth factors that have paracrine effects on surrounding cells. These growth factors include vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), and others.3 Growth factors have various functions. In a transgenic mouse model, VEGF has been proven to affect hair growth and follicular size by angiogenesis.4 Hepatocyte growth factor and IGF also activate hair growth through various pathways.5,6 Platelet-derived growth factor induces and maintains anagen hair in murine hair follicles.7 These observations suggest that ADSCs may have a therapeutic effect on hair loss. Indeed, we previously reported that ADSCs and the conditioned media of ADSCs (ADSC-CM) promote hair growth in vitro.8,9Hair Regeneration Treatment Using Adipose-Derived Stem CeLL

Female pattern hair loss (FPHL) is a very common type of alopecia seen in women. Its prevalence is approximately 6% in women aged under 50 years and 38% in women aged 70 years and over.10 Although FPHL rarely progresses to the total hair loss seen in male androgenetic alopecia, hair loss is more distressing to women than men because women with FPHL have a more negative body image in comparison with balding men.11 Nevertheless, FPHL patients have fewer therapeutic options than male androgenetic alopecia patients. Lower percentage topical minoxidil is the only medication approved by the US Food and Drug Administration for FPHL. Anti-androgen drugs and topical estrogen are used for the treatment of FPHL. However, they do not always achieve successful results. Anti-androgens and finasteride carry a risk for the feminizing of a male fetus. These treatments are therefore not safe in reproductive women. Topical estrogen still lacks convincing clinical trials.12 There is still a significant need for more effective therapy for FPHL. This study reports on our evaluation of outcomes in 27 patients in whom ADSC-CM was used to treat FPHL.

 

MATERIALS AND METHODS

Subjects

The medical records of FPHL patients were reviewed to collect subjects treated with only ADSC-CM at the dermatologic clinic (Cellpark Clinic, Seoul, South Korea) from January to October 2012. We excluded subjects who had used any products or drugs that might have influenced hair growth during the six months prior to ADSC-CM therapy. The patients had all been informed about the treatment they would receive. This study was approved by the Institutional Review Board of Seoul National University Hospital.Hair Regeneration Treatment Using Adipose-Derived Stem CeLL

Materials

The authors used the commercial ADSC-CM product AAPE (now known as NGAL) (Prostemics Research Institute, Sungnam, South Korea). AAPE is produced from human subcutaneous adipose tissue that is obtained by medical liposuction from healthy persons who provide informed consent. AAPE is manufactured by the method described below.9 Adipose tissues were exposed to 0.075% type II collagenase (Sigma-Aldrich Corp., St Louis, MO, USA) for 30 minutes at culture temperature, centrifuged at 400 g for 10 minutes, and washed and resuspended in phosphate-buffered saline (PBS). The stromal cell fraction was filtered through a 70-lm cell strainer (BD Biosciences, Inc., San Jose, CA, USA). Using Histopaque-1077 (Sigma-Aldrich Corp.), ADSCs were isolated from the filtrate and cultured at 37 °C in 5% carbon dioxide in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS). Characteristic expression levels of stem cell-related surface markers were confirmed by flow cytometry. ADSCs expressed CD73, CD90, and CD105 and were lacking in CD34 and CD49d. Adipogenic, osteogenic, and chondrogenic differentiation was also checked by the conventional method.13 After the isolation of ADSCs, the cells were pooled. ADSCs were cultured and expanded in normal control medium and used in experiments at passage 4. Cells were finally frozen in aliquots using Cell FreezerTM (Genenmed, Inc., Seoul, South Korea) for future use. A frozen vial containing 1 9 106 cells was inoculated into culture medium containing 10% FBS. After repeated subculture to reach a density of 5 9 108 cells, the expanded ADSCs were introduced into Cell FactoryTM CF10 (Nalge Nunc International Corp., Rochester, NY, USA) in DMEM/F12 serum-free medium (WelGene, Inc., Taegu, Korea). Cultures were conducted under hypoxic conditions by providing 2% oxygen using a nitrogen gas supply in a humidified multichannel incubator for two weeks. The conditioned media were collected and microfiltered and total protein production quantitated. Finally, for fresh use, 4-ml vials containing equal protein concentrations were freeze-dried as a single lot sample preparation of AAPETM.

Hair Regeneration Treatment Using Adipose-Derived Stem CeLL

TREATMENT PROTOCOL

The authors set a 12-week protocol for the treatment of FPHL with ADSC-CM. The treatment course consisted of repeated applications of ADSC-CM once per week for 12 consecutive weeks. The scalp area was gently cleansed prior to the application of AAPE with a micro-needle roller (MRS-05; Union Medical Co. Ltd, Uijeongbu, South Korea) (Fig. 1). Routine phototrichographic images were taken of all patients at the first visit and at 12 weeks using a digital camera (Folliscope; Lead M Co. Ltd, Seoul, South Korea) at magnifications of 930 and 960. At the first visit, the area in the midline scalp was tattooed with a black dot, and a phototrichogram was taken with the dot positioned in the center of the image. A phototrichogram of the same area was taken after 12 weeks of treatment.